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Journal of Undergraduate Research

Keywords

dipyrenylpropane, fluorescence probe, propane

College

Life Sciences

Department

Physiology and Developmental Biology

Abstract

1,3-di(1-pyrenyl)propane(hereinafter dipyrenylpropane) has proven very useful as a fluorescence probe in determining fluidity and monitoring phase transitions in biological membrane systems.1 By virtue of its structure, dipyrenylpropane may fluoresce light in an excimer or a monomer configuration. Each configuration fluoresces light at a different wavelength, thus allowing us to monitor the excimer-to-monomer intensity ratio. The excimer form occurs when, as the light is absorbed, the pyrene branches come within close proximity, transferring energy and fluorescing light back at a different wavelength.2 The monomer configuration is defined by the lack of energy transfer and a consistent fluorescence wavelength. Therefore, as membrane fluidity is increased it allows the pyrene branches to come in closer contact and increases the excimer-to-monomer ratio. Consequently, a low excimer-to-monomer ratio would correspond with low fluidity. Modeling dipyrenylpropane and simulating its behavior could prove valuable in future studies of membrane fluidity.3

Included in

Physiology Commons

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