Journal of Undergraduate Research
Keywords
gene delection, scherichia coli, PhoB, phosphorylate
College
Life Sciences
Department
Microbiology and Molecular Biology
Abstract
Two component systems are an integral part of a bacterial cell. They consist of a histidine kinase and a response regulator. The histidine kinase is a receptor protein that signals from the outside of the cell to the inside. Once a ligand binds to the histidine kinase, it autophosphorylates on the inside of the cell and activates the response regulator by phosphorylating it. Once the response regulator is activated, it can then phosphorylate another protein of the cell and that protein can carry out its function. An example of a two component system is the Pho system. PhoB is a response regulator that is phosphorylated by PhoR, a histidine kinase. When PhoB is phosphorylated, it binds to a region of DNA called a promoter that activates the trascription of a gene called phoA. The PhoA protein is an alkaline phosphatase which takes up phosphate from the environment when phosphate is in low quantities.
Recommended Citation
Beames, Brian J. and McCleary, Dr. William R.
(2013)
"Creating A Gene Deletion in Scherichia coli,"
Journal of Undergraduate Research: Vol. 2013:
Iss.
1, Article 1315.
Available at:
https://scholarsarchive.byu.edu/jur/vol2013/iss1/1315