•  
  •  
 

Journal of Undergraduate Research

Keywords

insertion points, macrophage fas-induced apoptosis, mafia

College

Life Sciences

Department

Microbiology and Molecular Biology

Abstract

Without site direction, transgenes integrate randomly into the genome of target cells. The transgene used in macrophage fas-induced apoptosis (Mafia) mice was randomly incorporated and indications of multiple insertion sites were observed. Our goal in performing this study was to detect the exact insertion point of the transgene so as to ultimately develop a genetic test to verify expression of the transgene in Maifa mice. In Figure 1 the premise of our experimental design is explained. Polymerase Chain Reaction (PCR) is a scientific technique that is used to amplify a specific segment of DNA. To determine the insertion points of interest, inverse nested-set PCR was used to identify the sequence adjoining the 5’ end of the Mafia transgene.

Download

Included in

Microbiology Commons

Share

COinS