Journal of Undergraduate Research
Keywords
p-21 Ras protein, HT-29 cell line, garlic, allium stivum
College
Life Sciences
Department
Microbiology and Molecular Biology
Abstract
How does fresh garlic oil affect human colon cancer cells? HT-29 is a cell line of human colon cancer originally extracted from a primary tumor in 1964. 3-hydroxy-3- methylglutaryi-CoA reductase (HGMR) is an enzyme responsible for attaching a lipid group to the 21 kilodalton (kD) Ras protein (p2l Ras), a molecule involved in cell signaling. This attachment is essential for the movement and anchoring of Ras from the cytosol to the membrane, as well as for biological activity. Diallyl disulfide (DADS) is a specific chemical found in garlic which has been shown to inhibit this enzyme1 and the translocation of Ras to the membrane.2 I wanted to see if fresh garlic oil would have the same effect on the intracellular movement of Ras.
Recommended Citation
Smith, Jordan D. and Murray, Dr. Byron K.
(2013)
"INHIBITION OF P-21 RAS TRANSLOCATION OF HT-29 CELL LINE BY FRESH GARLIC (ALLIUM SATIVUM),"
Journal of Undergraduate Research: Vol. 2013:
Iss.
1, Article 1269.
Available at:
https://scholarsarchive.byu.edu/jur/vol2013/iss1/1269