Chemical Analysis of Synthetic Dimerizer AP20187

Authors

Jad Mills, Brigham Young University
Dr. Sandra Burnett, Brigham Young University

Keywords

chemical analysis, synthetic dimerizer, AP20187, FKBP

College

Life Sciences

Department

Microbiology and Molecular Biology

Abstract

The synthetic dimerizer AP20187 initiates targeted dimerization of mutant FK506 binding proteins (FKBP) to study cell functions, cell signaling pathways, and protein functions. In Macrophage Fas-Induced Apoptosis (Mafia) transgenic mice, AP20187 is used to initiate Macrophage apoptosis . To determine the mechanism behind some depletion variability in Mafia mice, AP20187 was characterized and degradation was monitored using hydrogen and carbon nuclear magnetic resonance (1H- and 13C-NMR), high performance liquid chromatography (HPLC), electro spray mass spectrometry (ESMS), polarimetry, and spectrophotometry.

Recommended Citation

Mills, Jad and Burnett, Dr. Sandra (2013) "Chemical Analysis of Synthetic Dimerizer AP20187," Journal of Undergraduate Research: Vol. 2013: Iss. 1, Article 1255.
Available at: https://scholarsarchive.byu.edu/jur/vol2013/iss1/1255