A Higher Resolution Multiplexed Microsatellite Marker set for Reliable, Cost Effective Genotyping

Authors

Robert J. Kolts, Brigham Young University
Elliott G. Richards, Brigham Young University
Sam Ashby, Brigham Young University
Will D. Brubaker, Brigham Young University
Aaron C. Ferguson, Brigham Young University
Paul Fjeldsted, Brigham Young University
Derek Hatch, Brigham Young University
Dr. Edward R. Wilcox, Brigham Young University

Keywords

genomewide, microsatellite marker, genetic disorders

College

Life Sciences

Department

Biology

Abstract

We are creating a new microsatellite marker set for public use. Because many projects involving genetic disorders in humans depend on microsatellite markers, there is an existing need for a reliable and cost-effective marker set that provides higher-quality statistical data while still being affordable. Our proposed set will consist of approximately 800 markers and currently has a rough spacing of one marker every 4.1 cM. The average heterozygosity is currently 0.85. To further reduce costs to users, we are arranging markers and corresponding PCR primers into ~178 multiplexed groups consisting of 4-5 markers each. These multiplexed groups will allow research teams to perform genomewide scans at minimal cost.

Recommended Citation

Kolts, Robert J.; Richards, Elliott G.; Ashby, Sam; Brubaker, Will D.; Ferguson, Aaron C.; Fjeldsted, Paul; Hatch, Derek; and Wilcox, Dr. Edward R. (2013) "A Higher Resolution Multiplexed Microsatellite Marker set for Reliable, Cost Effective Genotyping," Journal of Undergraduate Research: Vol. 2013: Iss. 1, Article 1059.
Available at: https://scholarsarchive.byu.edu/jur/vol2013/iss1/1059