The Effect of Celecoxib on 17-Estradiol (E2) Treated MCF-7 Cells Using the Enzyme Linked Immunoabsorbent Assay (ELISA)

Authors

Jason Kinyon, Brigham Young University

Keywords

celecoxib, 17b-Estradiol, E2, MCF-7 cells, immunoabsorbent assay

College

Life Sciences

Department

Biology

Abstract

There has been evidence that shows inhibiting Cyclo-oxygense 2 (COX-2) enzymes may decrease angiogenic factors such as Vascular Endothelial Growth Factor (VEGF), which may prove beneficial in halting angiogenesis and metastasis, stopping cancer progression. It has been shown that there is a significant positive correlation between COX-2 and VEGF mRNA expression in breast cancer specimens. 17b-Estradiol (E2) has shown to increase VEGF production by binding to estrogen receptor positive breast cancer cell lines such as MCF-7. The COX-2 inhibitor celecoxib can be added to E2 treated MCF-7 cells to determine its effect on VEGF production using the Enzyme Linked Immunoabsorbent Assay (ELISA).

Recommended Citation

Kinyon, Jason (2013) "The Effect of Celecoxib on 17-Estradiol (E2) Treated MCF-7 Cells Using the Enzyme Linked Immunoabsorbent Assay (ELISA)," Journal of Undergraduate Research: Vol. 2013: Iss. 1, Article 1017.
Available at: https://scholarsarchive.byu.edu/jur/vol2013/iss1/1017