Keywords

anti-inflammatory cytokines, colon adenocarcinoma, IL-10, Immunohistochemistry, metastatic colonadenocarcinoma, TGF-β

Abstract

Classical anti-inflammatory cytokines are known to play a role in both cancer progression as well as cancer elimination. We evaluated the anti-inflammatory cytokines IL-10 and TGF-β in patients with colon adenocarcinoma and metastatic colon adenocarcinoma using immunohistochemical assays to determine the expression of the cytokines between various malignant tissues. We found tissues stained with TGF-β showed no significant upregulation within malignant tumors when compared with normal tissue controls. We observed high levels of TGF-β presence in most tissues similar to GAPDH expression. Within both colon adenocarcinoma and metastatic carcinomas there was a significant variability among patients in the expression of IL-10. While some patients experienced insignificant increases in the cytokine compared with controls, other patients had a clear upregulation of the protein within their tissue. In addition, there was an increase in the number of patients positive for IL-10 upregulation within metastatic tumors when compared with primary tumors. These data indicate that there is substantial variability between patients in regards to IL-10 expression, which may further aid in characterizing tumors and evaluating metastatic potential.

Original Publication Citation

Townsend MH, Felsted AM, Piccolo SR, Robison RA, and O’Neill KL. Metastatic colon adenocarcinoma has a significantly elevated expression of IL-10 compared to primary colon adenocarcinoma tumors. Cancer Biology & Therapy; 2017 Aug 16:0. doi: 10.1080/15384047.2017.1360453

Document Type

Peer-Reviewed Article

Publication Date

2018-05-10

Publisher

Taylor and Francis Group

Language

English

College

Life Sciences

Department

Biology

University Standing at Time of Publication

Associate Professor

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