The determination of thiamin and its derivatives in brain tissue of control, thiamin-deficient, oxythiamin- and pyrithiamin-treated rats
The determination of total thiamin, free thiamin, thiamin di phosphate (TDP) plus thiamin triphosphate (TTP), and total α- hydroxyethylthiamin (HET) levels in rat brain in control, deficient, oxythiamin- (OTH) and pyrithiamin- (PTH) treated rats was accomplished. It was found that the TDP + TTP/thiamin ratio observed in the thiamin-deficient, OTH- and PTH-treated rats was constant and did not differ from the ratio observed in the control rat brains. The brain levels of TDP + TTP decreased to 39% and 12% of the control thiamin levels in deficient and PTH-treated rats respectively. The brain HET and TDP + TTP levels of the OTH-treated rats were not significantly different from the controls. The HET levels in the PTH rats decreased significantly ( α = 0.005) by treatment day 4 and decreased to one-seventh of the control values in the terminal stages. A significant drop in the HET levels from the control levels was interpreted to mean that the pyruvate utilization was significantly impaired in the brain.
College and Department
Chemistry and Biochemistry
BYU ScholarsArchive Citation
Murdock, David S., "The determination of thiamin and its derivatives in brain tissue of control, thiamin-deficient, oxythiamin- and pyrithiamin-treated rats" (1973). Theses and Dissertations. 8311.
Vitamin B1 deficiency, Rats