The role of thiamine pyrophosphate in regulating the level of gamma-aminobutyric acid in the central nervous system
The level of gamma-aminobutyric acid in the brains of white rats suffering from thiamine deficiency symptoms resulting from pyrithiamine or oxythiamine antagonism of thiamine or from thiamine deprivation was measured. The level of this amino acid was 17.5% lower in the brains of pyrithiamine-treated rats and 20.0% lower in the brains of thiamine-deprived rats than in the control rats. Daily injections of ten times the required amounts of vitamin B6 in rats treated with pyrithiamine or oxythiamine or by thiamine deprivation had no effect on the level of brain gamma-aminobutyric acid or on the psiological behavior of these rats. The activity of glutamic decarboxylase was assayed and found to be 29.0% lower in the brains of thiamine-deprived rats than in the controls. This enzyme was not significantly altered in pyrithiamine-treated or in oxythiamine-treated rats. The activity of glutamic-gamma-aminobutyric transaminase with succinic semialdehyde dehydrogenase in the brains of any of these groups was not significantly different than the activity in the control animals.
College and Department
Physical and Mathematical Sciences; Chemistry and Biochemistry
BYU ScholarsArchive Citation
Adams, Bryant L., "The role of thiamine pyrophosphate in regulating the level of gamma-aminobutyric acid in the central nervous system" (1965). Theses and Dissertations. 8149.
Vitamin B1, Amino acids