Hemoglobin, from the erythrocytes of adult and fetal pigs, was isolated and analyzed by carboxymethylcellulose column chromatography and electrophoresis on polyacetate. These techniques demonstrate the existence of four fetal components (F0, F1A, F1B, F1C) and two adult components (A0, A1) based on their elution pH values and electrophoretic mobilities. The shift from-fetal to adult forms is initiated sometime between eight weeks after birth and one year. The change is not completed until sometime after one year and the major fetal forms (F0, F1A) maintain a 15% level in a mature adult. This report is in contrast with the results of early work that indicated that pig hemoglobin was homogeneous in nature. It conforms to the work by other researchers on other animals by showing that pig hemoglobin is heterogeneous.
College and Department
Life Sciences; Plant and Wildlife Sciences
BYU ScholarsArchive Citation
Green, Donald William, "An electrophoretic and chromatographic study of the hemoglobin from adult and fetal pigs" (1967). Theses and Dissertations. 7936.
Hemoglobin; Electrophoresis; Chromatographic analysis; Swine