Preterm birth (PTB) is defined as birth before the 37th week of pregnancy and affects 15 million infants per year. Presently, there is no clinical test to determine PTB risk. A 3D printed microfluidic device is being developed as a clinical test for PTB risk via detection of a panel of biomarkers. A significant step is extraction of the PTB biomarkers from blood serum. In this work, I developed 3D printed microfluidic devices in which monoliths can be polymerized. Using the monolith as a solid support to attach antibody, I show that ferritin, one of the PTB biomarkers, can be selectively extracted from human blood serum. This is the first study where a monolith has been formed in a 3D printed microfluidic device and used to perform an immunoaffinity extraction. This work is an important step in developing a clinical test for PTB risk. The realization of this work also demonstrates that 3D printing can be used to fabricate functional microfluidic devices.
College and Department
Physical and Mathematical Sciences; Chemistry and Biochemistry
BYU ScholarsArchive Citation
Parker, Ellen Kelsey, "Development of Affinity Monoliths in 3D Printed Microfluidic Devices for Extraction of Preterm Birth Biomarkers" (2018). Theses and Dissertations. 7440.
3D printing, microfluidics, affinity monolith, preterm birth