Bio-filtration, Plasmid, DNA, Borosilicate, Filters, separation, swinnex, glass filter, ion exchange, affinity, chromotography
This research has been completed to determine whether plasmid DNA will bind to a simple borosilicate filter in a reusable filter holder, and whether it can be released in a controlled procedure after a wash has been done to eliminate impurities.
The motivation for this is to help small research groups, both in educational settings as well as in other small research settings be able to study isolated DNA without much of the expense generally associated with purification kits and to remove the limitations on trial numbers imposed by the expense of each individual isolation kit.
Medical research, forensics, and other biological studies often require the examination or multiplication of DNA. In order to examine the DNA, it must be separated from the other cellular components, or lysosomes. Many techniques have been developed by research groups to accomplish the goal of DNA isolation, including through Chromatography. This is any method that uses a stationary phase and a mobile phase. Some types of chromatography include Affinity Chromatography, Size Exclusion Chromatography and Ion Exchange Chromatography.
BYU ScholarsArchive Citation
Floyd, Stacey D.; Baird, Camille S.; Finnell, Jordan; Tsang, Tsz Ming; Davis, Robert C.; and Christensen, Kenneth A., "Plasmid DNA Bind and Elute Chromatography Using Borosilicate Filters and Filter Holders" (2016). All Student Publications. 193.
Physical and Mathematical Sciences
Physics and Astronomy
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