Description

Sepsis is a dysregulated immune response to circulating bacteria/endotoxin. Hemoperfusion is a promising treatment, but is not universally effective and can unacceptable blood damage. We are developing protein-repellent hydrogel nanofilms that can be grown on a variety of surfaces, and functionalized with “click-ready” pathogen-binding proteins expressed by Genetic Code Expansion (GCE). The extremely rapid, bioorthogonal “click” immobilization chemistry eliminates prior purification, maximizes surface loading, and guarantees spatial orientation of the immobilized proteins. We have demonstrated pathogen binding and blood protein repulsion by these layers, suggesting that they may be part of effective treatments for sepsis and other applications.

 

Towards a Bioselective Surface for Treatment of Sepsis in a Hemoperfusion Device

Sepsis is a dysregulated immune response to circulating bacteria/endotoxin. Hemoperfusion is a promising treatment, but is not universally effective and can unacceptable blood damage. We are developing protein-repellent hydrogel nanofilms that can be grown on a variety of surfaces, and functionalized with “click-ready” pathogen-binding proteins expressed by Genetic Code Expansion (GCE). The extremely rapid, bioorthogonal “click” immobilization chemistry eliminates prior purification, maximizes surface loading, and guarantees spatial orientation of the immobilized proteins. We have demonstrated pathogen binding and blood protein repulsion by these layers, suggesting that they may be part of effective treatments for sepsis and other applications.

 

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